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recombinant mouse mpo  (R&D Systems)


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    R&D Systems recombinant mouse mpo
    Recombinant Mouse Mpo, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mpo/Recombinant+Mouse+Myeloperoxidase+Protein%2C+CF/pm41507363-154-36-39
    Average 94 stars, based on 17 article reviews
    recombinant mouse mpo - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: The potentiation of myeloperoxidase activity by the glycosaminoglycan-dependent binding of myeloperoxidase to proteins of the extracellular matrix.
    Article Snippet: a Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno, Czech Republic b Department of Internal Medicine, University of California, Davis, CA, USA c International Clinical Research Center, Center of Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Brno, Czech Republic d Department of Animal Physiology, Faculty of Science, Masaryk University, Czech Republic e Department of Cardiology, University Heart Center Hamburg, University Hospital Eppendorf, Hamburg, Germany f Centre for Free Radical Research, Department of Pathology, University of Otago, Christchurch, New Zealand g Department of Physiology and Membrane Biology, University of California, Davis, CA, USA

    Recombinant:

    Article Title: The potentiation of myeloperoxidase activity by the glycosaminoglycan-dependent binding of myeloperoxidase to proteins of the extracellular matrix.
    Article Snippet: a Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno, Czech Republic b Department of Internal Medicine, University of California, Davis, CA, USA c International Clinical Research Center, Center of Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Brno, Czech Republic d Department of Animal Physiology, Faculty of Science, Masaryk University, Czech Republic e Department of Cardiology, University Heart Center Hamburg, University Hospital Eppendorf, Hamburg, Germany f Centre for Free Radical Research, Department of Pathology, University of Otago, Christchurch, New Zealand g Department of Physiology and Membrane Biology, University of California, Davis, CA, USA

    Article Title: Myeloperoxidase transforms chromatin into neutrophil extracellular traps.
    Article Snippet: .. HeLA mononucleosomes or recombinant biotinylated nucleosomes (Epicypher, 16-0002 (HeLa mononucleosomes), 16-0006 (recombinant wild type) and 16-0027 (recombinant tailless)) were co-incubated with either recombinant MPO (RnD systems) or native MPO (Sigma-Aldrich) at a molar ratio of 1 nucleosome to 0.5 rMPOs or 0.25 nMPOs for 5 min at room temperature in chromatin remodelling buffer (12 mM HEPES, 40 mM Tris-HCl, 0.32 mM EDTA, 3 mM MgCl2, 10% glycerol, 0.02% Igepal, 60 mM KCl and indicated NaCl concentrations, pH 7.4) and then directly loaded into native gels to monitor binding of MPO to nucleosome after the addition of native sample buffer (Invitrogen). ..

    Article Title: Myeloperoxidase acts as a profibrotic mediator of atrial fibrillation
    Article Snippet: We treated male C57BL/6J (WT) and MPO tm1lus mice ( Mpo −/− , Jackson Laboratory) on a C57BL/6 background, aged 12–15 weeks, via ALZET osmotic pumps with AngII (1.5 ng per g body weight per min, Sigma) or saline (vehicle) for 14 d before electrophysiological investigation. .. We treated another cohort of WT and Mpo −/− mice via ALZET osmotic pumps with a connected jugular catheter with recombinant MPO (3174-MP-250, R&D Systems, 5.0, 12.5 and 50 pg per g body weight per min, which we calculated to generate physiological plasma concentrations of MPO ranging between 100 and 1100 pM) or recombinant human serum albumin (Sigma) for 7 d in combination with either AngII or saline before electrophysiological investigation. ..

    Article Title: Myeloperoxidase transforms chromatin into neutrophil extracellular traps
    Article Snippet: .. HeLA mononucleosomes or recombinant biotinylated nucleosomes (Epicypher, 16-0002 (HeLa mononucleosomes), 16-0006 (recombinant wild type) and 16-0027 (recombinant tailless)) were co-incubated with either recombinant MPO (RnD systems) or native MPO (Sigma-Aldrich) at a molar ratio of 1 nucleosome to 0.5 rMPOs or 0.25 nMPOs for 5 min at room temperature in chromatin remodelling buffer (12 mM HEPES, 40 mM Tris-HCl, 0.32 mM EDTA, 3 mM MgCl 2 , 10% glycerol, 0.02% Igepal, 60 mM KCl and indicated NaCl concentrations, pH 7.4) and then directly loaded into native gels to monitor binding of MPO to nucleosome after the addition of native sample buffer (Invitrogen). ..

    Article Title: Myeloperoxidase aggravates pulmonary arterial hypertension by activation of vascular Rho-kinase
    Article Snippet: Male C57Bl/6J (WT) and MPO_tm1lus ( Mpo –/– ) mice aged 9–12 weeks were anesthetized with isoflurane (Abbott) and received buprenorphin (Essex-Pharma) for analgesia. .. An osmotic minipump (1007D, Alzet) filled with recombinant MPO (R&D Systems) and connected to an Alzet mouse jugular catheter (polyurethane, OD 0.36 mm) was implanted s.c. ..

    Article Title: Myeloperoxidase aggravates pulmonary arterial hypertension by activation of vascular Rho-kinase.
    Article Snippet: Male C57Bl/6J (WT) and MPO_tm1lus (Mpo–/–) mice aged 9–12 weeks were anesthetized with isoflurane (Abbott) and received buprenorphin (Essex-Pharma) for analgesia. .. An osmotic minipump (1007D, Alzet) filled with recombinant MPO (R&D Systems) and connected to an Alzet mouse jugular catheter (polyurethane, OD 0.36 mm) was implanted s.c. ..

    Article Title: Oxidized Phospholipids and Neutrophil Elastase Coordinately Play Critical Roles in NET Formation
    Article Snippet: Images were acquired using a Keyence BZ-X710 fluorescent microscope and images were analyzed using the BZ-X analyzer. .. Thirty micrograms of Egg PC (Avanti Polar Lipids) adjusted with PBS was incubated with recombinant MPO (R&D System) in the presence of 5 mM hydrogen peroxide for 60 min at 37°C. .. Then, lipid peroxidation of the sample was quantified using a TBARS Assay Kit (Cayman).

    Concentration Assay:

    Article Title: The potentiation of myeloperoxidase activity by the glycosaminoglycan-dependent binding of myeloperoxidase to proteins of the extracellular matrix.
    Article Snippet: a Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno, Czech Republic b Department of Internal Medicine, University of California, Davis, CA, USA c International Clinical Research Center, Center of Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Brno, Czech Republic d Department of Animal Physiology, Faculty of Science, Masaryk University, Czech Republic e Department of Cardiology, University Heart Center Hamburg, University Hospital Eppendorf, Hamburg, Germany f Centre for Free Radical Research, Department of Pathology, University of Otago, Christchurch, New Zealand g Department of Physiology and Membrane Biology, University of California, Davis, CA, USA

    Enzyme-linked Immunosorbent Assay:

    Article Title: The potentiation of myeloperoxidase activity by the glycosaminoglycan-dependent binding of myeloperoxidase to proteins of the extracellular matrix.
    Article Snippet: a Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno, Czech Republic b Department of Internal Medicine, University of California, Davis, CA, USA c International Clinical Research Center, Center of Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Brno, Czech Republic d Department of Animal Physiology, Faculty of Science, Masaryk University, Czech Republic e Department of Cardiology, University Heart Center Hamburg, University Hospital Eppendorf, Hamburg, Germany f Centre for Free Radical Research, Department of Pathology, University of Otago, Christchurch, New Zealand g Department of Physiology and Membrane Biology, University of California, Davis, CA, USA

    Binding Assay:

    Article Title: Myeloperoxidase transforms chromatin into neutrophil extracellular traps.
    Article Snippet: .. HeLA mononucleosomes or recombinant biotinylated nucleosomes (Epicypher, 16-0002 (HeLa mononucleosomes), 16-0006 (recombinant wild type) and 16-0027 (recombinant tailless)) were co-incubated with either recombinant MPO (RnD systems) or native MPO (Sigma-Aldrich) at a molar ratio of 1 nucleosome to 0.5 rMPOs or 0.25 nMPOs for 5 min at room temperature in chromatin remodelling buffer (12 mM HEPES, 40 mM Tris-HCl, 0.32 mM EDTA, 3 mM MgCl2, 10% glycerol, 0.02% Igepal, 60 mM KCl and indicated NaCl concentrations, pH 7.4) and then directly loaded into native gels to monitor binding of MPO to nucleosome after the addition of native sample buffer (Invitrogen). ..

    Article Title: Myeloperoxidase transforms chromatin into neutrophil extracellular traps
    Article Snippet: .. HeLA mononucleosomes or recombinant biotinylated nucleosomes (Epicypher, 16-0002 (HeLa mononucleosomes), 16-0006 (recombinant wild type) and 16-0027 (recombinant tailless)) were co-incubated with either recombinant MPO (RnD systems) or native MPO (Sigma-Aldrich) at a molar ratio of 1 nucleosome to 0.5 rMPOs or 0.25 nMPOs for 5 min at room temperature in chromatin remodelling buffer (12 mM HEPES, 40 mM Tris-HCl, 0.32 mM EDTA, 3 mM MgCl 2 , 10% glycerol, 0.02% Igepal, 60 mM KCl and indicated NaCl concentrations, pH 7.4) and then directly loaded into native gels to monitor binding of MPO to nucleosome after the addition of native sample buffer (Invitrogen). ..

    Clinical Proteomics:

    Article Title: Myeloperoxidase acts as a profibrotic mediator of atrial fibrillation
    Article Snippet: We treated male C57BL/6J (WT) and MPO tm1lus mice ( Mpo −/− , Jackson Laboratory) on a C57BL/6 background, aged 12–15 weeks, via ALZET osmotic pumps with AngII (1.5 ng per g body weight per min, Sigma) or saline (vehicle) for 14 d before electrophysiological investigation. .. We treated another cohort of WT and Mpo −/− mice via ALZET osmotic pumps with a connected jugular catheter with recombinant MPO (3174-MP-250, R&D Systems, 5.0, 12.5 and 50 pg per g body weight per min, which we calculated to generate physiological plasma concentrations of MPO ranging between 100 and 1100 pM) or recombinant human serum albumin (Sigma) for 7 d in combination with either AngII or saline before electrophysiological investigation. ..

    Saline:

    Article Title: Myeloperoxidase acts as a profibrotic mediator of atrial fibrillation
    Article Snippet: We treated male C57BL/6J (WT) and MPO tm1lus mice ( Mpo −/− , Jackson Laboratory) on a C57BL/6 background, aged 12–15 weeks, via ALZET osmotic pumps with AngII (1.5 ng per g body weight per min, Sigma) or saline (vehicle) for 14 d before electrophysiological investigation. .. We treated another cohort of WT and Mpo −/− mice via ALZET osmotic pumps with a connected jugular catheter with recombinant MPO (3174-MP-250, R&D Systems, 5.0, 12.5 and 50 pg per g body weight per min, which we calculated to generate physiological plasma concentrations of MPO ranging between 100 and 1100 pM) or recombinant human serum albumin (Sigma) for 7 d in combination with either AngII or saline before electrophysiological investigation. ..

    Incubation:

    Article Title: Oxidized Phospholipids and Neutrophil Elastase Coordinately Play Critical Roles in NET Formation
    Article Snippet: Images were acquired using a Keyence BZ-X710 fluorescent microscope and images were analyzed using the BZ-X analyzer. .. Thirty micrograms of Egg PC (Avanti Polar Lipids) adjusted with PBS was incubated with recombinant MPO (R&D System) in the presence of 5 mM hydrogen peroxide for 60 min at 37°C. .. Then, lipid peroxidation of the sample was quantified using a TBARS Assay Kit (Cayman).



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    Creative BioMart active recombinant mouse mpo
    ( A ) Schematic of splenocyte transfer model. ( B – E ) Splenocytes from <t>MPO</t> –/– mice immunized with <t>recombinant</t> mouse MPO (rmMPO) ( B , n = 3) or HA-rmMPO ( D , n = 3) caused glomerulonephritis, indicated by crescent formation (arrow) (scale bar: 20 μm). Splenocytes from WT mice immunized with rmMPO ( C , n = 3) or HA-rmMPO ( E , n = 3) did not cause glomerulonephritis in Rag2-knockout mice after transfer. ( F ) Quantification of glomerular crescents. ( G ) Quantification of glomerular necrosis. Data are expressed as mean ± SEM. Graph was drawn using GraphPad Prism (GraphPad Software, Version 9.5.1). *** P < 0.001; **** P < 0.0001 assessed by 1-way ANOVA with multiple comparisons.
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    R&D Systems ls002006 mouse myeloperoxidase mpo
    ( A ) Schematic of splenocyte transfer model. ( B – E ) Splenocytes from <t>MPO</t> –/– mice immunized with <t>recombinant</t> mouse MPO (rmMPO) ( B , n = 3) or HA-rmMPO ( D , n = 3) caused glomerulonephritis, indicated by crescent formation (arrow) (scale bar: 20 μm). Splenocytes from WT mice immunized with rmMPO ( C , n = 3) or HA-rmMPO ( E , n = 3) did not cause glomerulonephritis in Rag2-knockout mice after transfer. ( F ) Quantification of glomerular crescents. ( G ) Quantification of glomerular necrosis. Data are expressed as mean ± SEM. Graph was drawn using GraphPad Prism (GraphPad Software, Version 9.5.1). *** P < 0.001; **** P < 0.0001 assessed by 1-way ANOVA with multiple comparisons.
    Ls002006 Mouse Myeloperoxidase Mpo, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Schematic of splenocyte transfer model. ( B – E ) Splenocytes from MPO –/– mice immunized with recombinant mouse MPO (rmMPO) ( B , n = 3) or HA-rmMPO ( D , n = 3) caused glomerulonephritis, indicated by crescent formation (arrow) (scale bar: 20 μm). Splenocytes from WT mice immunized with rmMPO ( C , n = 3) or HA-rmMPO ( E , n = 3) did not cause glomerulonephritis in Rag2-knockout mice after transfer. ( F ) Quantification of glomerular crescents. ( G ) Quantification of glomerular necrosis. Data are expressed as mean ± SEM. Graph was drawn using GraphPad Prism (GraphPad Software, Version 9.5.1). *** P < 0.001; **** P < 0.0001 assessed by 1-way ANOVA with multiple comparisons.

    Journal: The Journal of Clinical Investigation

    Article Title: Sequential carbonyl derivatives and hydrazone adduct formation on myeloperoxidase contribute to development of ANCA vasculitis

    doi: 10.1172/JCI178813

    Figure Lengend Snippet: ( A ) Schematic of splenocyte transfer model. ( B – E ) Splenocytes from MPO –/– mice immunized with recombinant mouse MPO (rmMPO) ( B , n = 3) or HA-rmMPO ( D , n = 3) caused glomerulonephritis, indicated by crescent formation (arrow) (scale bar: 20 μm). Splenocytes from WT mice immunized with rmMPO ( C , n = 3) or HA-rmMPO ( E , n = 3) did not cause glomerulonephritis in Rag2-knockout mice after transfer. ( F ) Quantification of glomerular crescents. ( G ) Quantification of glomerular necrosis. Data are expressed as mean ± SEM. Graph was drawn using GraphPad Prism (GraphPad Software, Version 9.5.1). *** P < 0.001; **** P < 0.0001 assessed by 1-way ANOVA with multiple comparisons.

    Article Snippet: Active recombinant mouse MPO (Cat MPO-428M) was purchased from Creative BioMart Inc.

    Techniques: Recombinant, Knock-Out, Software